Prevalence of EPIYA motifs in Helicobacter pylori strains isolated from patients with gastroduodenal disorders in northern Iran

Authors

  • Abolghasem Ajami Molecular and cell biology cente , Faculty of medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Ahad Alizadeh Department of Biostatistics, Faculty of Health, Tehran University of Medical Sciences, Tehran, Iran
  • Alireza Rafiei Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  • Amin TalebiBezmin Abadi Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  • Mojtaba Shadman Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  • Vahid Hosseini Department of Internal Medicine, Imam Hospital, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  • Zahra Hosseini-khah Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Abstract:

Background: Cytotoxin-associated gene A (CagA)-positive strains of Helicobacter pylori are associated with gastroduodenal diseases. Evidences have suggested that the type of H pylori CagA EPIYA motifs may be associated with specific disorders (i.e., gastritis, peptic ulcer, or gastric cancer).  We investigated the prevalence of different EPIYA motifs (A, B, C, or D) in H. pylori strains isolated from patients with recurrent dyspepsia who underwent upper gastrointestinal (GI) endoscopy. Materials and Methods: H. pylori strains were isolated from biopsy specimens of 220 patients with dyspepsia symptoms. The presence of glmM gene, as a housekeeping gene CagA and pattern of cagA EPIYA motifs were genotyped using by polymerase chain reaction (PCR) method.The association between the type of motifs and disease state determined by the Chi-square test, Fisher’s exact test, and logistic regression. Results: CagA-positive H. pylori were identified in 125(57%) of patients with gastrointestinal disorders, of whom 36(28.6%) were isolated from gastritis, 31 (24.6%) from duodenal ulcer, and 58 (46.4%) from gastric cancer. The frequency of pattern of cagA EPIYA motifs were 39 (31.2%) AB motifs, 54 (43.2%) ABC motifs, and 32 (25.6%) ABCC motifs and not detected D motifs. Gastric cancer risk was estimated to be 2.57 times higher in patients infected by strains with ABCC motif when compared with gastritis and duodenal ulcer patients (P=0.03). Moreover, patients with C- containing motifs were 2.27 times more likely to be affilicted with gastric cancer than duodenal ulcer. AB motif was more associated with gastritis and duodenal ulcer than ABC and ABCC motifs. Conclusion: The results suggested that cag-EPIYA ABCC might be associated with gastric cancer, while EPIYA-AB might be is associated with duodenal ulcer.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Prevalence of Pathogenic Genes cagA and vacA of Helicobacter pylori Isolated in Patients with Digestive Disorders

Background and Aims: Helicobacter pylori is the main cause of various gastroduodenal diseases. It is estimated that app roximately, more than half of the adult population in developed countries and 90% of people in developing countries infected with H. pylori. H. pylori infection may be related to Genetic of virulence factors and environmental factors. The aim of this study was to assess of fre...

full text

High Prevalence of Helicobacter pylori hopQ II Genotype Isolated from Iranian Patients with Gastroduodenal Disorders

Helicobacter pylori plays an important role in the pathogenesis of chronic gastritis, peptic ulceration, and noncardia gastric cancer. Several putative virulence factors for H. pylori have been identified including vacA, babA, and iceA. HopQ is one of the outer membrane proteins involved in bacterial adherence to gastric mucosa and has been suggested to also play a role in the virulence of H. p...

full text

Prevalence of Coinfection Helicobacter pylori with Non-Helicobacter pylori Helicobacters Species in Patients Suffering from Gastric Diseases in Iran

Introduction: In addition to Helicobacter pylori, non-Helicobacter pylori helicobacters (NHPHs) have been diagnosed in the humans stomach that caused gastrointestinal diseases. This study aimed to evaluate the coinfection of H. pylori with NHPHs species in patients with gastric disorders in Iran.   Materials & Methods: This cross-sectional study was performed on 421 gastric biopsies form dysp...

full text

Discrimination of Dominant H. pylori Strains Isolated from Patients with Different Gastroduodenal Pathologies by Protein Profiling

It is not clear what factors determine divergent outcomes of infections caused by H. pylori . In the present study, the protein profiles of different strains of H. pylori, isolated from three groups of patients with ulcerative disease, non-ulcerative gastritis and cancer disease, were analyzed using 1DSDS-PAGE. The patterns of different H. pylori strains were highly divergent. About 30.76% (7 b...

full text

study of the caga gene prevalence in helicobacter pylori strains isolated from patients with upper gastrointestinal disorders in iran

introduction and aims: helicobacter pylori commonly is associated with gastritis: but only sometimes it causes clinically significant diseases such as gastric and duodenal ulcer. the development of disease depends on the virulence of the infecting h. pylori strain, the susceptibility of the host, and environment co-factors. the cytotoxin associated protein encoded by caga gene is an important v...

full text

Prevalence of cagA EPIYA motifs in Helicobacter pylori among dyspeptic patients in northeast Thailand.

The aims of this study were to determine the prevalence of cagA type in Helicobacter pylori isolated from dyspeptic patients in northeastern Thailand and to determine whether the pattern of cagA EPIYA motifs were associated with clinical outcomes. One hundred and forty-seven H. pylori-infected dyspeptic patients were enrolled, of whom 68 had non-ulcer dyspepsia (NUD), 57 peptic ulcer disease (P...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 1  issue 1

pages  29- 34

publication date 2013-02

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023